Journal: Frontiers in Immunology

Article Title: Dietary yeast glycoprotein supplementation improves the growth performance, intestinal health and disease resistance of largemouth bass ( Micropterus salmoides ) fed low-fishmeal diets

doi: 10.3389/fimmu.2023.1164087

Figure Lengend Snippet: Primers used for quantitative RT-PCR (qPCR).

Article Snippet: The primary antibodies containing P-TOR (#2971; CST, Danvers, MA, United States), TOR (#2972; CST, Danvers, MA, United States), P-S6 Ser235/236 (#4858; CST, Danvers, MA, United States), S6 (#2217; CST, Danvers, MA, United States).

Techniques: Quantitative RT-PCR, Sequencing

Journal: Frontiers in Immunology

Article Title: Dietary yeast glycoprotein supplementation improves the growth performance, intestinal health and disease resistance of largemouth bass ( Micropterus salmoides ) fed low-fishmeal diets

doi: 10.3389/fimmu.2023.1164087

Figure Lengend Snippet: Effects of dietary supplementation with YG on protein synthesis metabolism in the liver of largemouth bass. (A) Protein and phosphorylation levels of TOR and s6 in the liver. (B) Quantification of TOR protein phosphorylation. (C) Image quantification results of s6 protein phosphorylation. (D) Relative mRNA expression of tor . (E) Relative mRNA expression of s6 . (F) Relative mRNA expression of eif4e . (G) Relative mRNA expression of 4ebp2 . Columns represent the mean ± SEM (n = 6). For each index, bars not sharing a common letter indicate significant differences ( P < 0.05).

Article Snippet: The primary antibodies containing P-TOR (#2971; CST, Danvers, MA, United States), TOR (#2972; CST, Danvers, MA, United States), P-S6 Ser235/236 (#4858; CST, Danvers, MA, United States), S6 (#2217; CST, Danvers, MA, United States).

Techniques: Phospho-proteomics, Expressing

Journal: Journal of Nutritional Science

Article Title: Dietary supplementation with glutamate improves the flesh quality of gibel carp ( Carassius gibelio ) by altering muscle texture characteristics and increasing the deposition of flavour substances

doi: 10.1017/jns.2025.10009

Figure Lengend Snippet: Effects of Glu supplementation in the practical diets on protein and mRNA expression related to protein synthesis in dorsal muscle of gibel carp. (a) AKT and S6 protein phosphorylation in dorsal muscle were measured by western blot. GAPDH was used as a loading control ( n = 3). (b) The relative protein expression of p-AKT/AKT. (c) The relative protein expression of p-S6/S6. (d) The relative mRNA expression of protein synthesis in dorsal muscle. ‘*’ showed significantly differences between groups ( P < 0.05).

Article Snippet: Then, the membranes were incubated in anti-phospho-AKT antibodies (1:1000, #9272; CST, Danvers, MA, United States), anti-AKT antibodies (1:1000, #4060; CST, Danvers, MA, United States), anti-phospho-ribosomal protein s6 (S6) antibodies (1:1000, #4858; CST, Danvers, MA, United States), anti-S6 antibodies (1:1000, #2217; CST, Danvers, MA, United States), and anti-GAPDH antibodies (1:1000, ab8245; Abcam).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control

Journal: Molecular Neurobiology

Article Title: GSK3β Controls mTOR and Prosurvival Signaling in Neurons

doi: 10.1007/s12035-017-0823-9

Figure Lengend Snippet: Primary antibodies

Article Snippet: P-S6 ribosomal protein (S235/S236) , Cell Signaling Technology (Danvers, MA, USA) , 4858 , Rabbit , 1:1000 (WB) 1:150 (IF).

Techniques: